姬松茸提取物组分体外抗病毒感染活性的研究     

樊廷俊  钱冠兰  杜玉堂  袁文鹏  吕雅萌 《中国海洋大学学报(自然科学版)》2008,38(5)

为了探明姬松茸提取物的体外抗病毒感染活性及其作用方式,进而为水产养殖鱼类高效抗淋巴囊肿病毒(lymphocystis disease virus,LCDV)感染活性物质的开发和鲆蝶类淋巴囊肿病的防治奠定基础,利用热水浸提和酒精沉淀法得到了5种姬松茸提取物组分(E1～5),并利用MTT、细胞病变程度观察等方法研究了5种组分对LCDV感染体外培养大菱鲆鳍细胞(turbot fin cells,TF细胞)的影响作用.细胞毒性实验结果显示,5种组分对体外培养TF细胞均无毒性.细胞病变程度结果表明,本文所得5种姬松茸提取物组分尤其是E5组分具有显著的抗LCDV感染TF细胞的活性.不同方式感染的实验结果进一步显示,5种姬松茸提取物组分抗LCDV感染TF细胞的作用可能主要是通过直接灭活病毒和/或阻断病毒吸附细胞来实现的.  相似文献   

Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses     

YOU Feng  WANG Wei  XU Dongdong  ZHU Xiangping  NI Jing  WU Zhihao  XU Yongli  WANG Xincheng  ZHANG Peijun 《中国海洋湖沼学报》2009,27(2)

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder Sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes,12 confirmed hybridization of the paternal and matemal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.  相似文献   

Grey relational analysis on the relation between marine environmental factors and oxidation-reduction potential   总被引：2，自引：0，他引：2  

刘学庆  王佳  张盾  李言涛 《中国海洋湖沼学报》2009,27(3):583-586

The effects of marine environmental factors-temperature(T),dissolved oxygen(DO),salinity(S) and pH-on the oxidation-reduction potential(ORP) of natural seawater were studied in laboratory.The results show an indistinct relationship between these four factors and the ORP,but they did impact the ORP.Common mathematical methods were not applicable for describing the relationship.Therefore,a grey relational analysis(GRA) method was developed.The degrees of correlation were calculated according to GRA and the va...  相似文献   

基于改进形态学运算的图像去噪自适应算法研究     

许致火  何建新  朱幼莲 《成都信息工程学院学报》2009,24(1)

形态学的腐蚀和膨胀两种基本运算固有特征,使其在去除图像噪声的同时,造成图像信息严重丢失.针对这种现象,首先把传统腐蚀膨胀的极值运算改进为中值运算;其次对结构元素进行改进和优化,定义单位结构元素,利用数学方法推导找到零方阵的单位结构元素;最后在这些研究工作的基础上提出一种新的自适应图像去噪算法.对比实验表明:新方法比传统形态学和中值滤波算法在低信噪比环境下具有明显优势,因此在图像去噪中具有广泛的应用前景.  相似文献   

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赵建虎  刘辉  王胜平 《大地测量与地球动力学》2009,29(2):82-87

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贺凯飞  徐天河 《大地测量与地球动力学》2009,29(6):109-112

?????GEO????????????????????????????????????Kalman???????GEO?????????????????????????????????????η????????????????????????????????????????????????????????????????????????????????????з?????????????????????????????????????????Kalman???????????н????????????????к???????????????????????,????????????????Kalman????????????????????????????,???????Kalman???????????????????????????????????????????Kalman?????????????????????GEO?????????????  相似文献   

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刘宁  张永志  吴小利  刘锋 《大地测量与地球动力学》2009,29(4):30-33

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郭琳  刘玉光  刘雪源  吕学珠  李轶斐 《大地测量与地球动力学》2009,29(4):64-69

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戴伟  焦文海  李维鹏  贾小林 《大地测量与地球动力学》2009,29(4):111-115

?????????????????￡????ú?????????GPS Block IIR??M??????????????????????о??????????GM(1??1)??AR(p)??????????1???????????????1 ns????????10??????????????10 ns??????????????ζ??????GM??1??1???????????????????????IGS(the International GPS Service for Geodynamics)??????????7 ns??????  相似文献   

Immunotoxicity effect of benzo[α]pyrene on scallop Chlamys farreri     

Lin Zhang  Luqing Pan  Jing Liu 《中国海洋大学学报(英文版)》2009,8(1):89-94

The toxic effects of benzo[α]pyrene (B[α]P) at different concentrations (0.1, 0.5, 1, 2.5 and 7.5 μgL⁻¹) on scallop (Chlamy farreri) immune system were studied. The results showed that B[α]P had significant toxic effects on the haemocyte counts, neutral red uptake, phagocytosis, bacteriolytic and antibacterial activity (P<0.05), while the seawater control and acetone control had no significant differences. The haemocyte counts, neutral red uptake, phagocytosis and bacteriolytic activity in all B[α]P treatment groups as well as antibacterial activity in groups of 0.5, 1, 2.5 and 7.5 μgL⁻¹ B[α]P decreased significantly (P<0.05). Some of these indices tended to be stable on the sixth day and others on the ninth day, and the indices showed clear time- and concentration-response to B[α]P. Bacteriolytic activity in 0.1μgL⁻¹ B[α]P treatment group and antibacterial activity in 0.1 μgL⁻¹ and 0.5 μgL- B[α]P treatment groups increased at the beginning of exposure and reached their peaks on day 1 and day 6, respectively. Following that, both activities decreased gradually and became stable after day 9. When all the indices reached stability, they were significantly lower than those in control group (P<0.05), except for antibacterial activity in 0.1 μgL⁻¹ B[α]P treatment group (P>0.05). Thus, B[α] has evident toxic effects on scallop immune system, which supports the view that a relationship exists between pollution and immunomodulation in aquatic organisms.  相似文献